PolyQ-expanded ataxin-3 protein levels in peripheral blood mononuclear cells correlate with clinical parameters in SCA3: a pilot study

PolyQ-expanded ataxin-3 protein levels in peripheral blood mononuclear cells correlate with clinical parameters in SCA3: a pilot study

In view of upcoming scientific trials, quantitative molecular markers accessible in peripheral blood are of important significance as prognostic or pharmacodynamic markers in genetic neurodegenerative ailments comparable to Spinocerebellar Ataxia Kind 3 (SCA3), specifically for signaling goal engagement. On this pilot examine, we centered on the quantification of ataxin-3, the protein altered in SCA3, in human peripheral blood mononuclear cells (PBMCs) acquired from preataxic and ataxic SCA3 mutation carriers in addition to wholesome controls, as a molecular marker immediately associated to SCA3 pathophysiology.

 

We established two completely different extremely delicate TR-FRET-based immunoassays to measure the protein ranges of both complete full-length, non-expanded and expanded, ataxin-Three or particularly polyQ-expanded ataxin-3. In PBMCs, a transparent discrimination between SCA3 mutation service and controls had been seen measuring polyQ-expanded ataxin-Three protein stage. Moreover, polyQ-expanded ataxin-Three protein ranges correlated with illness development and scientific severity as assessed by the Scale for the Evaluation and Score of Ataxia. Complete full-length ataxin-Three protein ranges had been immediately influenced by the expression ranges of the polyQ-expanded ataxin-Three protein, however weren’t correlated with scientific parameters. Evaluation of ataxin-Three ranges in fibroblasts or induced pluripotent stem cells allowed to differentiate mutation carriers from controls, thus offering proof-of-principle validation of our PBMC findings throughout cell traces.

Complete full-length or polyQ-expanded ataxin-Three protein was not detectable by TR-FRET assays in different biofluids like plasma or cerebrospinal fluid, indicating the necessity for ultra-sensitive assays for these biofluids. Standardization research revealed that tube techniques, blood sampling, and PBMC preparation could affect ataxin-Three protein ranges indicating a excessive demand for standardized protocols in biomarker research. In conclusion, the polyQ-expanded ataxin-Three protein is a promising candidate as a molecular goal engagement marker in SCA3 in future scientific trials, determinable even in-easily accessible-peripheral blood biomaterials. These outcomes, nevertheless, require validation in a bigger cohort and additional standardization of modifying situations.

 

 

Capturing the Conformational Ensemble of the Blended Folded Polyglutamine Protein Ataxin-3

Ataxin-Three is a deubiquitinase concerned in protein high quality management and different important mobile capabilities. It preferentially interacts with polyubiquitin chains of 4 or extra items hooked up to proteins delivered to the ubiquitin-proteasome system. Ataxin-Three consists of an N-terminal Josephin area and a versatile C terminus that comprises two or three ubiquitin-interacting motifs (UIMs) and a polyglutamine tract, which, when expanded past a threshold, results in protein aggregation and misfolding and causes spinocerebellar ataxia kind 3. The high-resolution construction of the Josephin area is offered, however the structural and dynamical heterogeneity of ataxin-Three has to this point hindered the structural description of the full-length protein.
Right here, we characterize non-expanded and expanded variants of ataxin-Three by way of conformational ensembles adopted by the proteins in resolution by collectively utilizing experimental knowledge from nuclear magnetic resonance and small-angle X-ray scattering with coarse-grained simulations. Our outcomes pave the best way to a molecular understanding of polyubiquitin recognition. Right here, we developed an immunoassay that readily detects polyQ ATXN3 proteins in human organic fluids and discriminates sufferers with SCA3 from wholesome controls and people with different ataxias. We present that polyQ ATXN3 serves as a marker of goal engagement in human fibroblasts, which can bode properly for its use in scientific trials.
Final, we recognized a single-nucleotide polymorphism that strongly associates with the expanded allele, thus offering an thrilling drug goal to abrogate detrimental occasions initiated by mutant ATXN3. Gene-silencing methods for a number of repeat ailments are properly underneath method, and our outcomes are anticipated to enhance scientific trial preparedness for SCA3 therapies.
 PolyQ-expanded ataxin-3 protein levels in peripheral blood mononuclear cells correlate with clinical parameters in SCA3: a pilot study

PolyQ-expanded ataxin-3 protein levels in peripheral blood mononuclear cells correlate with clinical parameters in SCA3: a pilot study

Dynamics of a Protein Interplay Community Related to the Aggregation of polyQ-Expanded Ataxin-1

Background: A number of experimental fashions of polyglutamine (polyQ) ailments have been beforehand developed which can be helpful for learning illness development within the primarily affected central nervous system. Nevertheless, there’s a lacking hyperlink between mobile and animal fashions that may point out the molecular defects occurring in neurons and are chargeable for the illness phenotype in vivo.
Strategies: Right here, we used a computational method to establish dysregulated pathways shared by an in vitro and an in vivo mannequin of ATXN1(Q82) protein aggregation, the mutant protein that causes the neurodegenerative polyQ illness spinocerebellar ataxia type-1 (SCA1).
Outcomes: A set of widespread dysregulated pathways had been recognized, which had been utilized to assemble cerebellum-specific protein-protein interplay (PPI) networks at numerous time-points of protein aggregation. Evaluation of a SCA1 community indicated essential nodes which regulate its perform and may characterize potential pharmacological targets. Moreover, a set of medicine interacting with these nodes and predicted to enter the blood-brain barrier (BBB) was recognized.

Ataxin 3 protein

30R-1344 50 ug
EUR 305
Description: Purified recombinant Human Ataxin 3 protein

Ataxin 3 antibody

22437-100ul 100ul
EUR 390

Ataxin 3 antibody

70R-13630 100 ul
EUR 457
Description: Affinity purified Rabbit polyclonal Ataxin 3 antibody

Ataxin-3 Protein

20-abx262861
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

Ataxin 3 Polyclonal Antibody

27857-100ul 100ul
EUR 252

Ataxin 3 Polyclonal Antibody

27857-50ul 50ul
EUR 187

Recombinant Human Ataxin-3

7-04462 2µg Ask for price

Recombinant Human Ataxin-3

7-04463 10µg Ask for price

Recombinant Human Ataxin-3

7-04464 1mg Ask for price

Ataxin 3 Rabbit pAb

A1243-100ul 100 ul
EUR 308

Ataxin 3 Rabbit pAb

A1243-200ul 200 ul
EUR 459

Ataxin 3 Rabbit pAb

A1243-20ul 20 ul
EUR 183

Ataxin 3 Rabbit pAb

A1243-50ul 50 ul
EUR 223

Ataxin 3 Rabbit pAb

A12992-100ul 100 ul
EUR 308

Ataxin 3 Rabbit pAb

A12992-200ul 200 ul
EUR 459

Ataxin 3 Rabbit pAb

A12992-20ul 20 ul
EUR 183

Ataxin 3 Rabbit pAb

A12992-50ul 50 ul
EUR 223

Ataxin-3 (pS256) Antibody

abx148457-100ug 100 ug
EUR 439
  • Shipped within 5-10 working days.

Ataxin-3 (Phospho-Ser256) Antibody

12574-100ul 100ul
EUR 252

Ataxin-3 (Phospho-Ser256) Antibody

12574-50ul 50ul
EUR 187

Ataxin 3 / ATX3 (ATXN3) Antibody

20-abx111106
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul
  • Shipped within 5-10 working days.

Ataxin 3 / ATX3 (ATXN3) Antibody

abx036768-100ug 100 ug
EUR 391
  • Shipped within 5-10 working days.

Ataxin 3 / ATX3 (ATXN3) Antibody

abx038360-100ug 100 ug
EUR 391
  • Shipped within 5-10 working days.

Ataxin 3 Polyclonal Conjugated Antibody

C27857 100ul
EUR 397

Ataxin 3 / ATX3 (ATXN3) Antibody

20-abx318334
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Ataxin 3 / ATX3 (ATXN3) Antibody

20-abx225054
  • EUR 370.00
  • EUR 606.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 50 ul
  • Shipped within 5-10 working days.

Phospho-Ataxin-3 (Ser256) Antibody

AF8242 200ul
EUR 376
Description: Ataxin-3 (Phospho-Ser256) Antibody detects endogenous levels of Ataxin-3 only when phosphorylated at Ser256.

Ataxin- 3 (Phospho- Ser256) Antibody

ABF8242 100 ug
EUR 438

Ataxin 3 / ATX3 (ATXN3) Antibody

20-abx001155
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

Anti-Ataxin 3/ATXN3 Antibody

PA1846 100ug/vial
EUR 334

Anti-Ataxin 3/ATXN3 Antibody

PB9423 100ug/vial
EUR 334

Human Ataxin 3(ATXN3) ELISA kit

E01A1769-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human Ataxin 3(ATXN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Ataxin 3(ATXN3) ELISA kit

E01A1769-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human Ataxin 3(ATXN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Ataxin 3(ATXN3) ELISA kit

E01A1769-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human Ataxin 3(ATXN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Ataxin 3(ATXN3) ELISA kit

E02A1769-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Ataxin 3(ATXN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Ataxin 3(ATXN3) ELISA kit

E02A1769-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Ataxin 3(ATXN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Ataxin 3(ATXN3) ELISA kit

E02A1769-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Ataxin 3(ATXN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Ataxin 3(ATXN3) ELISA kit

E06A1769-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat Ataxin 3(ATXN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Ataxin 3(ATXN3) ELISA kit

E06A1769-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat Ataxin 3(ATXN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Ataxin 3(ATXN3) ELISA kit

E06A1769-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat Ataxin 3(ATXN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Ataxin 3(ATXN3) ELISA kit

E03A1769-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Mouse Ataxin 3(ATXN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Ataxin 3(ATXN3) ELISA kit

E03A1769-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Mouse Ataxin 3(ATXN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Ataxin 3(ATXN3) ELISA kit

E03A1769-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Mouse Ataxin 3(ATXN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Ataxin 3(ATXN3) ELISA kit

E04A1769-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Ataxin 3(ATXN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Ataxin 3(ATXN3) ELISA kit

E04A1769-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Ataxin 3(ATXN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Ataxin 3(ATXN3) ELISA kit

E04A1769-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Ataxin 3(ATXN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Ataxin 3(ATXN3) ELISA kit

E07A1769-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Porcine Ataxin 3(ATXN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Ataxin 3(ATXN3) ELISA kit

E07A1769-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Porcine Ataxin 3(ATXN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Ataxin 3(ATXN3) ELISA kit

E07A1769-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Porcine Ataxin 3(ATXN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Ataxin 3(ATXN3) ELISA kit

E08A1769-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Canine Ataxin 3(ATXN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Ataxin 3(ATXN3) ELISA kit

E08A1769-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Canine Ataxin 3(ATXN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Ataxin 3(ATXN3) ELISA kit

E08A1769-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Canine Ataxin 3(ATXN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Ataxin 3(ATXN3) ELISA kit

E09A1769-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Monkey Ataxin 3(ATXN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Ataxin 3(ATXN3) ELISA kit

E09A1769-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Monkey Ataxin 3(ATXN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Ataxin 3(ATXN3) ELISA kit

E09A1769-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Monkey Ataxin 3(ATXN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Ataxin- 3, Atxn3 ELISA KIT

ELI-11387m 96 Tests
EUR 865

Human Ataxin- 3, ATXN3 ELISA KIT

ELI-11466h 96 Tests
EUR 824

Chicken Ataxin- 3, ATXN3 ELISA KIT

ELI-24058c 96 Tests
EUR 928

Ataxin-3-Like Protein (ATXN3L) Antibody

abx230742-100ug 100 ug
EUR 481
  • Shipped within 5-12 working days.

Ataxin-3-Like Protein (ATXN3L) Antibody

abx230743-100ug 100 ug
EUR 509
  • Shipped within 5-12 working days.

Ataxin 3 / ATX3 (ATXN3) Antibody (HRP)

20-abx303178
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Ataxin 3 / ATX3 (ATXN3) Antibody (FITC)

20-abx303179
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Ataxin 3 / ATX3 (ATXN3) Antibody (Biotin)

20-abx303180
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Phospho-Ataxin-3 (Ser256) Blocking Peptide

AF8242-BP 1mg
EUR 195

ATXN3 Ataxin-3 Human Recombinant Protein

PROTP54252 Regular: 10ug
EUR 317
Description: ATXN3 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 370 amino acids (1-370 a.a.) and having a molecular mass of 42.4kDa.;ATXN3 is purified by proprietary chromatographic techniques.

Human Ataxin 3(ATXN3)ELISA Kit

QY-E01558 96T
EUR 361

Ataxin 2 antibody

70R-50365 100 ul
EUR 244
Description: Purified Polyclonal Ataxin 2 antibody

Ataxin 10 antibody

70R-50914 100 ul
EUR 244
Description: Purified Polyclonal Ataxin 10 antibody

Ataxin 1 Antibody

AF6347 200ul
EUR 304
Description: Ataxin 1 Antibody detects endogenous levels of total Ataxin 1.

Ataxin 1 Antibody

ABF6347 100 ug
EUR 438

Guinea pig Ataxin 3(ATXN3) ELISA kit

E05A1769-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Guinea pig Ataxin 3(ATXN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Ataxin 3(ATXN3) ELISA kit

E05A1769-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Guinea pig Ataxin 3(ATXN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Ataxin 3(ATXN3) ELISA kit

E05A1769-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Guinea pig Ataxin 3(ATXN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Ataxin-7-Like Protein 3 (ATXN7L3) Antibody

20-abx121023
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.

Ataxin-7-Like Protein 3 (ATXN7L3) Antibody

abx037189-100ug 100 ug
EUR 391
  • Shipped within 5-10 working days.

Human Ataxin 3 / ATX3 (ATXN3) ELISA Kit

abx259421-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.

Ataxin-3 (Phospho-Ser256) Polyclonal Conjugated Antibody

C12574 100ul
EUR 397

Ataxin-7-Like Protein 3 (ATXN7L3) Antibody

20-abx301379
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Ataxin 7 Like 3 (ATXN7L3) Antibody (HRP)

20-abx306773
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Ataxin 7 Like 3 (ATXN7L3) Antibody (FITC)

20-abx306774
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Ataxin 7 Like 3 (ATXN7L3) Antibody (Biotin)

20-abx306775
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Anti-14-3-3 alpha + beta Rabbit Monoclonal Antibody

M02431-3 100ug/vial
EUR 397
Description: Rabbit Monoclonal 14-3-3 alpha + beta Antibody. Validated in Flow Cytometry, IP, IF, IHC, ICC, WB and tested in Human, Mouse, Rat.

IL-3 Interleukin-3 Human Recombinant Protein, His Tag

PROTP08700-3 Regular: 50ug
EUR 317
Description: Interleukin-3 Human Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 154 amino acids fragment (20-152) and having a total molecular mass of 17.3kDa and fused with a 20 aa N-terminal His tag. ;The IL3 His is purified by proprietary chromatographic techniques.

Individual Reaction Mix 3

G065-3 200 reactions
EUR 167

3-D Life Thioglycerol

T10-3 180 µl
EUR 48

Western Blot Box - 2 7/8 x 1 3/16 x 3/4in.; 7.3 x 3 x 1.9cm

B1200-3 5/pack
EUR 63.77
Description: Western Blot Boxes

TGF-b-3 Transforming Growth Factor-Beta 3 Human Recombinant Protein, Plant

PROTP10600-3 Regular: 5ug
EUR 317
Description: TGFB3 Human Recombinant produced in plant is a disulfide-linked homodimeric, glycosylated, polypeptide chain containing 118 amino acids and having a molecular mass of 27.2kDa. ;The TGFB3 is fused to 6xHis tag at N-terminus and purified by standard chromatographic techniques.

Dr. P Kit-Solution 3

K2021010-3 50 ml
EUR 133
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

3-D Life PEG-Link

L50-3 3x 200 µl
EUR 94

3-D Life CD-Link

L60-3 3 x 200 µl
EUR 321

3-D-Life SG-PVA

M81-3 3x 170 µl
EUR 148

3-D Life FG-PVA

M82-3 3x 170 µl
EUR 148

3-D Life SG-Dextran

M91-3 3x 170 µl
EUR 157

3-D Life FG-Dextran

M92-3 3x 170 µl
EUR 157

3-D Life RGD Peptide

P10-3 3x 1 µmol
EUR 276

Recombinant Human MMP-3 Protein

PROTP08254-3 10ug
EUR 317
Description: Matrix metalloproteinases (MMPs) are a family of endoproteases that require zinc and calcium for expressing catalytic activity. These enzymes play a central role in the maintenance and remodeling of the extracellular matrix. Elevated expression of their activity, caused either by up-regulation of their expression or down-regulation of their cognate inhibitors, has been implicated in various degenerative disorders, including arthritis, cardiovascular disease, skeletal growth-plate disorders, and cancer metastasis. MMP-3 degrades fibronectin, laminin, collagens III, IV, and X, and cartilage proteoglycans. Recombinant human MMP-3 is a 42.8 kDa protein containing the entire catalytic N-terminal domain and the C-terminal domain (378 amino acids).

pLenti-CARM1 shRNA-3 Plasmid

PVTBAV03691-3 2 ug
EUR 356

pLenti-CTLA4 shRNA-3 Plasmid

PVTBAV05689-3 2 ug
EUR 356

pLenti-FOXM1 shRNA-3 Plasmid

PVTBAV08732-3 2 ug
EUR 356
Conclusions: Our examine factors to molecular mechanisms of SCA1 linked from each mobile and animal fashions and suggests medication that could possibly be examined to find out whether or not they have an effect on the aggregation of pathogenic ATXN1 and SCA1 illness development.

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