Mesenchymal stem cell-derived exosomes: Toward cell-free therapeutic

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Engineered targeting tLyp-1 exosomes as gene therapy vectors for efficient delivery of siRNA into lung cancer cells

Natural exosomes can express specific proteins and carbohydrate molecules on the surface and hence have demonstrated the great potentials for gene therapy of cancer. However, the use of natural exosomes is restricted by their low transfection efficiency.

Here, we report a novel targeting tLyp-1 exosome by gene recombinant engineering for delivery of siRNA to cancer and cancer stem cells. To reach such a purpose, the engineered tLyp-1-lamp2b plasmids were constructed and amplified in Escherichia coli. The tLyp-1-lamp2b plasmids were further used to transfect HEK293T tool cells and the targeting tLyp-1 exosomes were isolated from secretion of the transfected HEK293T cells.

Afterwards, the artificially synthesized siRNA was encapsulated into targeting tLyp-1 exosomes by electroporation technology. Finally, the targeting siRNA tLyp-1 exosomes were used to transfect cancer or cancer stem cells. Results showed that the engineered targeting tLyp-1 exosomes had a nanosized structure (approximately 100 nm) and high transfection efficiency into lung cancer and cancer stem cells.

The function verifications demonstrated that the targeting siRNA tLyp-1 exosomes were able to knock-down the target gene of cancer cells and to reduce the stemness of cancer stem cells. In conclusion, the targeting tLyp-1 exosomes are successfully engineered, and can be used for gene therapy with a high transfection efficiency. Therefore, the engineered targeting tLyp-1 exosomes offer a promising gene delivery platform for future cancer therapy.

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Exosome fluorescent staining

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Normal human exosome RNA

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ExoA375 100 μg
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Lyophilized Exosome Standards

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Description: Lyophilized Exosomes from HT-29, a human colon cancer cell line

Lyophilized Exosome Standards

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Exosome Component 9 Protein

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Exosome Component 8 Protein

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Exosome Component 1 Protein

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Exosome Component 1 Protein

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Exosome Component 1 (EXOSC1) Antibody

abx030303-80l 80 µl
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abx029390-400ul 400 ul
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abx232898-100ug 100 ug
EUR 577.2

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abx232901-100ug 100 ug
EUR 577.2

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abx232902-100ug 100 ug
EUR 661.2

Exosome Component 3 (EXOSC3) Antibody

abx232903-100ug 100 ug
EUR 577.2

Exosome Component 4 (EXOSC4) Antibody

abx232904-100ug 100 ug
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Exosome Component 5 (EXOSC5) Antibody

abx232905-100ug 100 ug
EUR 610.8

Exosome Component 7 (EXOSC7) Antibody

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EUR 661.2

Exosome Component 8 (EXOSC8) Antibody

abx232907-100ug 100 ug
EUR 577.2

Exosome Component 9 (EXOSC9) Antibody

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Exosome Component 5 (EXOSC5) Antibody

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Exosome Component 1 (EXOSC1) Antibody

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Exosome Component 8 (EXOSC8) Antibody

20-abx318649
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Exosome Component 3 (EXOSC3) Antibody

20-abx312069
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Exosome Component 8 (EXOSC8) Antibody

abx003377-100l 100 µl
EUR 400

Exosome Component 8 (EXOSC8) Antibody

abx003377-20l 20 µl
EUR 175

Exosome Component 8 (EXOSC8) Antibody

abx003377-50l 50 µl
EUR 275

Exosome Component 2 (EXOSC2) Antibody

abx172308-1ml 1 ml
EUR 787.5

Exosome Component 2 (EXOSC2) Antibody

abx176346-1096tests 10 × 96 tests
EUR 750

Exosome Component 2 (EXOSC2) Antibody

abx176346-596tests 5 × 96 tests
EUR 337.5

Exosome Component 2 (EXOSC2) Antibody

abx176346-96tests 96 tests
EUR 275

Exosome Component 1 (EXOSC1) Antibody

abx112409-100l 100 µl
EUR 612.5

Exosome Component 2 (EXOSC2) Antibody

abx112411-100l 100 µl
EUR 612.5

Exosome Component 3 (EXOSC3) Antibody

abx112412-100l 100 µl
EUR 612.5

Exosome Component 4 (EXOSC4) Antibody

abx112413-100l 100 µl
EUR 612.5

Exosome Component 5 (EXOSC5) Antibody

abx112414-100l 100 µl
EUR 612.5

Exosome Component 8 (EXOSC8) Antibody

abx112415-100l 100 µl
EUR 612.5

Exosome Component 1 (EXOSC1) Antibody

abx301763-100g 100 µg
EUR 362.5

Exosome Component 1 (EXOSC1) Antibody

abx301763-20g 20 µg
EUR 162.5

Exosome Component 1 (EXOSC1) Antibody

abx301763-50g 50 µg
EUR 250

Exosome Component 5 (EXOSC5) Antibody

abx302857-100g 100 µg
EUR 362.5

Exosome Component 5 (EXOSC5) Antibody

abx302857-20g 20 µg
EUR 162.5

Exosome Component 5 (EXOSC5) Antibody

abx302857-50g 50 µg
EUR 250

Exosome Component 2 (EXOSC2) Antibody

abx034983-100g 100 µg
EUR 281.25

Exosome Component 1 (EXOSC1) Antibody

abx030303-400l 400 µl
EUR 518.75

Exosome Component 8 (EXOSC8) Antibody

abx031706-400l 400 µl
EUR 518.75

 

Mesenchymal stem cell-derived exosomes: Toward cell-free therapeutic strategies in regenerative medicine

Mesenchymal stem cells (MSCs) are multipotent stem cells with marked potential for regenerative medicine because of their strong immunosuppressive and regenerative abilities. The therapeutic effects of MSCs are based in part on their secretion of biologically active factors in extracellular vesicles known as exosomes.

Exosomes have a diameter of 30-100 nm and mediate intercellular communication and material exchange. MSC-derived exosomes (MSC-Exos) have potential for cell-free therapy for diseases of, for instance, the kidney, liver, heart, nervous system, and musculoskeletal system. Hence, MSC-Exos are an alternative to MSC-based therapy for regenerative medicine. We review MSC-Exos and their therapeutic potential for a variety of diseases and injuries.

Exosomes derived from stem cells as an emerging therapeutic strategy for intervertebral disc degeneration

Intervertebral disc (IVD) degenerative diseases are a common problem in the world, and they cause substantial social and economic burdens for people. The current methods for treating IVD degenerative diseases mainly include surgery and conservative treatment, which cannot fundamentally restore the normal structure of the disc.

With continuous research on the mechanism of degeneration and the development of regenerative medicine, rapid progress has been made in the field of regenerative medicine regarding the use of stem cell-derived exosomes, which are active biological substances used in intercellular communication, because they show a strong effect in promoting tissue regeneration.

The study of exosomes in the field of IVD degeneration has just begun, and many surprising achievements have been made. This paper mainly reviews the biological characteristics of exosomes and highlights the current status of exosomes in the field of IVD degeneration, as well as future developments regarding exosomes.

Plasma-derived exosome-like vesicles are enriched in lyso-phospholipids and pass the blood-brain barrier

Exosomes are vesicles involved in intercellular communication. Their membrane structure and core content is largely dependent on the cell of origin. Exosomes have been investigated both for their biological roles and their possible use as disease biomarkers and drug carriers.

These potential technological applications require the rigorous characterization of exosomal blood brain barrier permeability and a description of their lipid bilayer composition. To achieve these goals, we have established a 3D static blood brain barrier system based on existing systems for liposomes and a complementary LC-MS/MS and 31P nuclear magnetic resonance methodology for the analysis of purified human plasma-derived exosome-like vesicles.

Results show that the isolated vesicles pass the blood brain barrier and are taken up in endothelial cells. The compositional analysis revealed that the isolated vesicles are enriched in lyso phospholipids and do not contain phosphatidylserine. These findings deviate significantly from the composition of exosomes originating from cell culture, and may reflect active removal by macrophages that respond to exposed phosphahtidylserine.

Human Exosome Component 1(EXOSC5)ELISA Kit

QY-E01127 96T
EUR 433.2

Human Exosome Component 1(EXOSC1)ELISA Kit

QY-E01131 96T
EUR 433.2

Human Exosome Component 1 (EXOSC1)ELISA Kit

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Human Exosome Component 1 (EXOSC1)ELISA Kit

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Exosome Component 1 Protein

20-abx263423
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Exosome Component 1 Protein

abx263423-1mg 1 mg
EUR 225

Exosome Component 1 Protein

abx263423-2mg 2 mg
EUR 325

Exosome Component 1 Protein

abx263423-5mg 5 mg
EUR 1600

Exosome Component 1 (EXOSC1) Antibody

20-abx301763
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Exosome Component 1 (EXOSC1) Antibody

20-abx125829
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Exosome Component 1 (EXOSC1) Antibody

abx232898-100ug 100 ug
EUR 577.2

Exosome Component 1 (EXOSC1) Antibody

20-abx112409
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Exosome Component 1 (EXOSC1) Antibody

abx030303-400ul 400 ul
EUR 627.6

Exosome Component 1 (EXOSC1) Antibody

abx030303-80l 80 µl
EUR 343.2

Exosome Component 1 (EXOSC1) Antibody

abx301763-100g 100 µg
EUR 362.5

Exosome Component 1 (EXOSC1) Antibody

abx301763-20g 20 µg
EUR 162.5

Exosome Component 1 (EXOSC1) Antibody

abx301763-50g 50 µg
EUR 250

Exosome Component 1 (EXOSC1) Antibody

abx125829-100l 100 µl
EUR 175

Exosome Component 1 (EXOSC1) Antibody

abx125829-1ml 1 ml
EUR 400

Exosome Component 1 (EXOSC1) Antibody

abx125829-200l 200 µl
EUR 275

Exosome Component 1 (EXOSC1) Antibody

abx112409-100l 100 µl
EUR 612.5

Exosome Component 1 (EXOSC1) Antibody

abx232898-100g 100 µg
EUR 350

Exosome Component 1 (EXOSC1) Antibody

abx030303-400l 400 µl
EUR 518.75

Recombinant Human Exosome Component 9

7-05902 2µg Ask for price

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7-05903 10µg Ask for price

Recombinant Human Exosome Component 9

7-05904 1mg Ask for price

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7-05905 5µg Ask for price

Recombinant Human Exosome Component 10

7-05906 20µg Ask for price

Recombinant Human Exosome Component 10

7-05907 1mg Ask for price

Human Exosome Component 1, EXOSC1 GENLISA ELISA

KBH2687 1 x 96 wells
EUR 286

Human Exosome Component 1, EXOSC5 GENLISA ELISA

KBH2691 1 x 96 wells
EUR 286

EXOSC1 (untagged)-Human exosome component 1 (EXOSC1)

SC114517 10 µg Ask for price

EXOSC1 (untagged)-Human exosome component 1 (EXOSC1)

SC322121 10 µg Ask for price

Human Exosome Component 2 ELISA Kit

IHUEXOSC2KT each
EUR 733
Description: Human Exosome Component 2 ELISA Kit

Exosome Component 5 Human Recombinant

rAP-3265 Inquiry Ask for price

Exosome Component 9 Human Recombinant

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Exosome Component 3 Human Recombinant

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Exosome Component 7 Human Recombinant

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Exosome Component 10 Human Recombinant

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Exosome Component 4 Human Recombinant

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Human Exosome Component 2 (EXOSC2) Protein

20-abx653328
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Human Exosome Component 2 (EXOSC2) Protein

abx653328-5mg 5 mg
EUR 337.5

Human anti exosome component 10(EXOSC10) antibody Elisa Kit

EK714539 96 Wells
EUR 0.46

Exosome Component 1 (EXOSC1) Antibody (HRP)

20-abx312486
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Exosome Component 1 (EXOSC1) Antibody (HRP)

abx312486-100g 100 µg
EUR 362.5

Exosome Component 1 (EXOSC1) Antibody (HRP)

abx312486-20g 20 µg
EUR 162.5

Exosome Component 1 (EXOSC1) Antibody (HRP)

abx312486-50g 50 µg
EUR 250

Human Exosome Component 2 (EXOSC2) CLIA Kit

20-abx495555
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Human Exosome Component 7 (EXOSC7) CLIA Kit

20-abx496063
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  • 96 tests

EXOSC1 (GFP-tagged) - Human exosome component 1 (EXOSC1)

RG206007 10 µg Ask for price

Exosome Component 1 (EXOSC1) Antibody (FITC)

20-abx312487
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Exosome Component 1 (EXOSC1) Antibody (FITC)

abx312487-100g 100 µg
EUR 362.5

Exosome Component 1 (EXOSC1) Antibody (FITC)

abx312487-20g 20 µg
EUR 162.5

Exosome Component 1 (EXOSC1) Antibody (FITC)

abx312487-50g 50 µg
EUR 250

EXOSC1 Exosome Component 1 Human Recombinant Protein

PROTQ9Y3B2 Regular: 10ug
EUR 380.4
Description: EXOSC1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 218 amino acids (1-195a.a) and having a molecular mass of 23.8kDa. ;EXOSC1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Human Exosome Component 2 (EXOSC2)ELISA Kit

201-12-2689 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Exosome Component 3 (EXOSC3)ELISA Kit

201-12-2690 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Exosome Component 4 (EXOSC4)ELISA Kit

201-12-2691 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Exosome Component 2 (EXOSC2) ELISA Kit

DLR-EXOSC2-Hu 96T
EUR 463
Description: tissue homogenates, cell lysates or other biological fluids.

Human Exosome Component 2 (EXOSC2) ELISA Kit

DLR-EXOSC2-Hu-48T 48T
EUR 620.4
Description: A sandwich quantitative ELISA assay kit for detection of Human Exosome Component 2 (EXOSC2) in samples from tissue homogenates, cell lysates or other biological fluids.

Human Exosome Component 2 (EXOSC2) ELISA Kit

DLR-EXOSC2-Hu-96T 96T
EUR 807.6
Description: A sandwich quantitative ELISA assay kit for detection of Human Exosome Component 2 (EXOSC2) in samples from tissue homogenates, cell lysates or other biological fluids.

Human EXOSC7(Exosome Component 7) ELISA Kit

ELK7511-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EXOSC7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EXOSC7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EXOSC7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EXOSC7 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human EXOSC7(Exosome Component 7) ELISA Kit

ELK7511-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EXOSC7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EXOSC7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EXOSC7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EXOSC7 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human EXOSC2(Exosome Component 2) ELISA Kit

ELK4639-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EXOSC2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EXOSC2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EXOSC2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EXOSC2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human EXOSC2(Exosome Component 2) ELISA Kit

ELK4639-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EXOSC2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EXOSC2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EXOSC2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EXOSC2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human Exosome Component 7 (EXOSC7) ELISA Kit

EKU09336-48T 48T
EUR 610.82

Human Exosome Component 7 (EXOSC7) ELISA Kit

EKU09336-5x96T 5x96T
EUR 4144.85

Human Exosome Component 7 (EXOSC7) ELISA Kit

EKU09336-96T 96T
EUR 872.6

Human Exosome Component 7 (EXOSC7) ELISA Kit

EKN50447-48T 48T
EUR 380.31

Human Exosome Component 7 (EXOSC7) ELISA Kit

EKN50447-5x96T 5x96T
EUR 2580.68

Human Exosome Component 7 (EXOSC7) ELISA Kit

EKN50447-96T 96T
EUR 543.3

Human Exosome Component 2 (EXOSC2) ELISA Kit

EKU03997-48T 48T
EUR 555.66

Human Exosome Component 2 (EXOSC2) ELISA Kit

EKU03997-5x96T 5x96T
EUR 3770.55

Human Exosome Component 2 (EXOSC2) ELISA Kit

EKU03997-96T 96T
EUR 793.8

Human Exosome Component 2 (EXOSC2) ELISA Kit

EKN45108-48T 48T
EUR 378.7

Human Exosome Component 2 (EXOSC2) ELISA Kit

EKN45108-5x96T 5x96T
EUR 2569.75

Human Exosome Component 2 (EXOSC2) ELISA Kit

EKN45108-96T 96T
EUR 541

Human Exosome Component 2 (EXOSC2) ELISA Kit

DL-EXOSC2-Hu 96T
EUR 441
Description: tissue homogenates, cell lysates or other biological fluids.

Human Exosome Component 2 (EXOSC2) ELISA Kit

EK13874 96Т
EUR 768

Human Exosome Component 3 (EXOSC3) ELISA Kit

abx387222-96tests 96 tests
EUR 1093.2

Human Exosome Component 4 (EXOSC4) ELISA Kit

abx387223-96tests 96 tests
EUR 1093.2

Human Exosome Component 5 (EXOSC5) ELISA Kit

abx387224-96tests 96 tests
EUR 1093.2

Human Exosome Component 8 (EXOSC8) ELISA Kit

abx387226-96tests 96 tests
EUR 1093.2

Human Exosome Component 9 (EXOSC9) ELISA Kit

abx387227-96tests 96 tests
EUR 1093.2

Human Exosome Component 7 (EXOSC7) ELISA Kit

20-abx258177
  • Ask for price
  • Ask for price
  • Ask for price
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Exosome Component 2 (EXOSC2) ELISA Kit

20-abx151457
  • Ask for price
  • Ask for price
  • Ask for price
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Exosome Component 2 (EXOSC2) ELISA Kit

RDR-EXOSC2-Hu-48T 48T
EUR 475.34
Description: tissue homogenates, cell lysates and other biological fluids.

Human Exosome Component 2 (EXOSC2) ELISA Kit

RDR-EXOSC2-Hu-48Tests 48 Tests
EUR 652.8

Human Exosome Component 2 (EXOSC2) ELISA Kit

RDR-EXOSC2-Hu-96T 96T
EUR 679.04
Description: tissue homogenates, cell lysates and other biological fluids.

Human Exosome Component 2 (EXOSC2) ELISA Kit

RDR-EXOSC2-Hu-96Tests 96 Tests
EUR 907.2

Human Exosome Component 2 (EXOSC2) ELISA Kit

RD-EXOSC2-Hu-48T 48T
EUR 452.7
Description: tissue homogenates, cell lysates and other biological fluids.

Human Exosome Component 2 (EXOSC2) ELISA Kit

RD-EXOSC2-Hu-48Tests 48 Tests
EUR 625.2

Human Exosome Component 2 (EXOSC2) ELISA Kit

RD-EXOSC2-Hu-96T 96T
EUR 646.7
Description: tissue homogenates, cell lysates and other biological fluids.

Human Exosome Component 2 (EXOSC2) ELISA Kit

RD-EXOSC2-Hu-96Tests 96 Tests
EUR 867.6

Human Exosome Component 2 ELISA Kit (EXOSC2)

RK01330 96 Tests
EUR 625.2

Human Exosome Component 7 ELISA Kit (EXOSC7)

RK01331 96 Tests
EUR 625.2

Human Exosome Component 4(EXOSC4)ELISA Kit

QY-E01128 96T
EUR 433.2