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UPX™ Universal Protein Extraction Kit


UPX™ Universal Protein Extraction KitProtein Extraction with Complete Solubilization

Extract Membrane Proteins and Soluble Proteins for Mass Spectrometry Analysis

UPX Universal Protein Extraction Kit is for researchers who need universal, unbiased extraction of both membrane proteins and soluble proteins, and an abundance  of starting material.

Extract Proteins at High Efficiency and Solubilize the Entire Proteome.

One Protein Extraction Protocol for Tissues and Cells, Fresh or Frozen.

UPX Protein Extraction Kit work with high efficiency on a wide variety of samples such as heart, liver, brain, and kidney organ tissues, mammalian-derived cultured cell lines, and bacteria. You can begin with freshly harvested or frozen cells.

Unbiased, High-Yield Extraction.

Compatible with SDS-PAGE, Gelfree® Fractionation, FASP™ Digestion

The lysis buffer in the UPX Protein Extraction Kit maximizes the extraction of proteins from cells and tissues under conditions that are compatible with downstream methods for preparing samples for mass spectrometry. Containing reducing agents and SDS, the lysis buffer provides unbiased, total extraction of membrane proteins and soluble proteins from cells and tissue. Solubulized proteins in the resulting lysates can be analyzed by SDS-PAGE and fractionated by the Gelfree 8100 Fractionation System. Recommends Filter-Aided Sample Preparation (FASP) for detergent depletion and complete trypsin digestion of extracts prior to analysis by mass spectrometry.