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FFPE-FASP™ Protein Digestion Kit

De-paraffinization and Uncrosslinking. Unbiased Extraction and Complete Solubilization. Same-As-Fresh Quantification.

FFPE Tissue Proteomics with FASP

Filter-Aided Sample Prep (FASP) is the enabling technology behind quantitative mass spectrometry analysis of archived tissues. Based on a spin-filter sample preparation method initially described by Manza, et al., and developed further and extended to FFPE tissue processing by Ostasiewicz, et al., this method features unbiased extraction, complete proteome solubilization, and highly efficient digestion. The resulting filtrate is free of detergents, large molecules, and other substances that would interfere with mass spectrometry analysis of the proteome.

Same-as-Fresh Quantification

FFPE tissue proteomes can now be quantified and analyzed by mass spectrometry. An extensive, quantitative mass spectrometry comparison study described by Ostasiewicz, et al. found no significant differences between the proteomes of fresh and FFPE tissue samples processed using FFPE-FASP. Proteins, peptides, and post-translational modifications were quantitatively preserved. Results were unaffected by hematoxylin staining, laser capture microdissection, or tissue storage time.