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FASP™ Protein Digestion Kit

Complete Protein Solubilization. Complete Trypsin Digestion. Every Time.


Universal Sample Preparation with the FASP Protein Digestion Kit

Now you can use aggressive, whole proteome solubilization protocols and reagents to prepare proteins from any biological material for trypsin digestion and analysis by mass spectrometry. Based on the Universal Sample Preparation method developed by Wisniewski, Zoubman, Nagaraj and Mann, FASP Protein Digestion Kit enables efficient digestion of samples for proteome analysis, even in the presence of the most extreme contaminating species. This method is the perfect complement to lysis buffers containing strong detergents and reducing agents. No longer are you forced to choose between efficient extraction or digestion. The FASP kit allows you to do both at the highest efficiency.

A Universal Method for Trypsin Digestion

Initially described by Manza, et al., and further developed by Wisniewski, et al., the concept of using a spin filter for cleanup and digestion of protein samples for mass spectrometry analyses offers several advantages over more conventional approaches. In FASP (Filter-Aided Sample Prep), the spin filter forms the basis of a “proteomic reactor” for sample preparation. This proteomic reactor is used to deplete SDS, salts, and other interfering substances from the protein sample prior to alkylation and trypsin digestion. Following digestion, peptides are eluted as a concentrated solution. High molecular weight interferences such as DNA remain trapped by the filter, so they stay out of the peptide solution filtrate.