GENTAUR Belgium

Tel +32 16 58 90 45
Fax +32 16 50 90 45
info@gentaur.com

Voortstraat 49,
1910 Kampenhout


GENTAUR France SARL

Tel 01 43 25 01 50
Fax 01 43 25 01 60
france@gentaur.com

9, rue Lagrange, 75005 Paris


GENTAUR GmbH

Tel tech support 0241 56 00 99 68
Frau Karolina Eland
or 0241 95 78 94 78
Mr Tryan Ilkov

Tel logistics
0241 40 08 90 86 Annick Verdeyen
Fax:
0241 55 91 05 36
de@gentaur.com

Marienbongard 20
52062 Aachen Deutschland


GENTAUR Poland

TEL Gdansk 058 710 33 44
FAX 058 710 33 48
(TEL production Warsaw
0223 98 82 21)

poland@gentaur.com

Sp. z o.o. PL2040002710
Ulica Ogarna 15/19B m2
80-826 GDANSK Poland


GENTAUR Italy

tel 02 36 00 65 93
fax 02 36 00 65 94
italia@gentaur.com

20135 Milano


GENTAUR Spain

tel:0911876558
spain@gentaur.com

FASP™ Protein Digestion Kit

Complete Protein Solubilization. Complete Trypsin Digestion. Every Time.

 

Universal Sample Preparation with the FASP Protein Digestion Kit

Now you can use aggressive, whole proteome solubilization protocols and reagents to prepare proteins from any biological material for trypsin digestion and analysis by mass spectrometry. Based on the Universal Sample Preparation method developed by Wisniewski, Zoubman, Nagaraj and Mann, FASP Protein Digestion Kit enables efficient digestion of samples for proteome analysis, even in the presence of the most extreme contaminating species. This method is the perfect complement to lysis buffers containing strong detergents and reducing agents. No longer are you forced to choose between efficient extraction or digestion. The FASP kit allows you to do both at the highest efficiency.

A Universal Method for Trypsin Digestion

Initially described by Manza, et al., and further developed by Wisniewski, et al., the concept of using a spin filter for cleanup and digestion of protein samples for mass spectrometry analyses offers several advantages over more conventional approaches. In FASP (Filter-Aided Sample Prep), the spin filter forms the basis of a “proteomic reactor” for sample preparation. This proteomic reactor is used to deplete SDS, salts, and other interfering substances from the protein sample prior to alkylation and trypsin digestion. Following digestion, peptides are eluted as a concentrated solution. High molecular weight interferences such as DNA remain trapped by the filter, so they stay out of the peptide solution filtrate.