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Proteoquant™ Proteome Quantification Assay Kit

Whole Proteome Quantification

Accurate BCA Assay Protein Quantification in the Presence of Reducing Agents

Proteoquant Proteome Quantification Assay Kit is for researchers using universal sample preparation methods to prepare whole proteome starting material for analysis. Based on a modification of the simple and scalable BCA protein quantification assay, the Proteoquant Proteome Quantification Assay Kit is compatible with the UPX Universal Protein Extraction Kit, the YPX Yeast Protein Extraction Kit, and other protocols for protein extraction into solutions containing both SDS and reducing agents. A protocol modification, included with the kit, allows researchers to omit the thiol alkylation step if their chosen extraction method does not require the presence of the reducing agents TCEP, DTT, or ϐ-mercaptoethanol in the extraction solution.

Using a modification of the traditional BCA assay method, the Proteoquant Proteome Quantification Assay Kit accurately quantifies proteins in the presence of reducing agents, without losing the high accuracy and easy scalability benefits of the original BCA assay:

Determine Protein Concentrations Accurately with or without TCEP

Determine Protein Concentrations Accurately with or without DTT

Determine Protein Concentrations Accurately with or without ϐME

The working range of the Proteoquant Proteome Quantification Assay Kit assay is 125 – 2000 μg/mL. When used in accord with Protocol 1 of the package insert, the assay tolerates the presence of up to 10 mM TCEP, 5 mM DTT, and 25 mM ϐ-mercaptoethanol without loss of accuracy.